A modular library allows you to build new genetic modules or pathways from basic DNA parts including regulatory elements (promoters, untranslated regions, signal peptides, terminators) and/or coding sequences. Multiple genetic elements can be combined in various predefined arrangements to create new transcriptional units, biochemical pathways, genetic circuits, or develop strains displaying new traits.
Examples of use: Use a Modular Library to easily test a coding sequence under the control of several different promoters, or assemble BioBrick modules such as promoter, ribosome binding site and terminator. Modularize protein function by building modular polyketide synthases for efficient synthesis of non-natural natural products. Use in conjunction with ProteinGPS™ to identify relevant modules.
- Size: 102
- Test a variety of modules in all possible permutations
- BioBricks compatible
- Integrated with Gene Designer for dynamic access to validated modules
Modular Libraries can be delivered as Linear DNA Fragments or Ligation Products. Linear DNA Fragments The fastest delivery at the lowest cost with the highest possible diversity, as no reduction of initial diversity has occurred through cloning, transformation, etc.. The DNA fragment pool is sequence verified (electronic trace files included) showing mixed peaks in the degenerate positions and single peaks in the non-degenerate positions. DNA2.0 can also clone a subset and individually sequence a selected number (typically 12-96, depending upon library complexity) to verify library diversity (preferred method). Library Cloned into Vector of Choice DNA2.0 will clone into the vector (library fragment insert ligated into vector of choice), allowing for multiple rounds of transformation in order to achieve the maximal number of screenable clones. DNA2.0 will transform and sequence a subset of clones (typically 12-96, depending upon library complexity) to verify the designed diversity distribution of the library. DNA2.0 can provide an array of the individually sequenced clones.
Genetic Engineering & Biotechnology News 2013. 33(8):18. Library Format for Bioengineering. Maximizing Screening Efficiency through Good Design. Levay-Young et al.
ACS Synthetic Biology 2013. Using Synthetic Biological Parts and Microbioreactors to Explore the Protein Expression Characteristics of Escherichia coli. Gorochowski et al.
PNAS 2009 106(14):5610-5. A family of thermostable fungal cellulases created by structure-guided recombination. Heinzelman, P. et al
Consult with a Library Design Specialist:
Contact DNA2.0 at +1 877 362 8646 or info@DNA20.com to determine the best possible option for your research project.
Custom Design Your Own Modular Library:
Use the DNA2.0 Library Designer to start your library today.
If your variant project is less complex than a library, just a few synthetic gene variants, see Variant Synthesis for how DNA2.0 can create the ideal strategy to move your research forward quickly and affordably.
When screening a large library becomes impractical, a rational, design of experiment (DoE) methodology can be used to create variant sets that thoroughly sample a defined set of substitutions in a minimal number of test genes. Learn more.