Gene Expression Solutions

Every gene and protein construct is different. Every research lab has unique protocols and methods. How do you know if your gene expression is optimal?

DNA2.0 has the experience and knowledge to tell you.

Expression Test: $395 USD per gene

Use the DNA2.0 Expression Test to quickly know what expression levels to expect with your synthetic gene. No more questioning if your gene was unable to express or if difficulties were due to protocols followed. DNA2.0 will clone and test your gene for expression levels in E. coli using our pJExpress 400 series of T5 vectors (401, 402, 404, 406) or certain E. coli Electra® vectors (pD441-SR and pD444-SR).

Deliverables:
  • DNA2.0 Expression Data Report
  • SDS-PAGE results for total and soluble protein fractions
  • Semi-quantitative Western (genes with His or Flag tag) results*
  • Growth and induction protocols

View typical results.

*See Terms & Conditions Tab

Guaranteed Expression: $750 USD per gene

We’re so confident of our gene design research and results that if you use our GeneGPS™ optimization algorithms, and your gene qualifies (see below for restrictions), DNA2.0 will guarantee that your synthetic gene will express.

DNA2.0 will clone and test your gene for expression levels in E. coli using our pJexpress 400 series of T5 vectors (401, 402, 404, 406). Should the construct not meet the expression standard*, DNA2.0 will send you a $750 USD GeneCard (details below).

Deliverables:
  • DNA2.0 Expression Data Report
  • SDS-PAGE results for total and soluble protein fractions
  • Semi-quantitative Western (genes with His or Flag tag) results*
  • Growth and induction protocols
  • DNA2.0 $750 USD GeneCard Guarantee (see below for details)

The fine print: Guaranteed Expression is valid only for qualifying genes. Gene must have been designed using DNA2.0 patented GeneGPS algorithms for optimization and meet DNA2.0 in silico analysis requirements. Examples for gene not being accepted for guarantee include: some membrane proteins, proteins known to be toxic to E. coli or very small proteins.

Should your gene fail to express, DNA2.0 will credit your account with a $750.00 USD DNA2.0 GeneCard. The GeneCard balance can be applied to future DNA2.0 orders. There are no fees returned for proteins which do not express.

View typical results.

*See Terms & Conditions Tab

Custom Solutions

Difficult Protein? Different expression hosts?

For a majority of proteins, genes designed using our patented E. coli Expression Algorithms result in high expression yields in E. coli. However, every protein is different, and some proteins contain features that resist initial expression attempts. If your gene is in this minority, DNA2.0 has the years of necessary experience to quickly and affordably overcome these obstacles.

DNA2.0 will alter the coding, add tags, edit promoters and adapt other relevant variables to avoid problems or improve desired characteristics such as toxicity, solubility, and organelle targeting.

DNA2.0 will make coding variants of your gene to optimize yields in your expression host of choice. Your gene will be optimized for maximal productivity in your system.

Fees vary based on your individual project needs. Consult with an Expression Specialist today at +1 877 DNA TOGO or info@DNA20.com.

Terms & Conditions

  • Prices are per gene and are in addition to regular gene synthesis fees.
  • Expression Test and Guaranteed Expression services must be placed at time of order for each gene.
  • * Expression Standard is >5µg/ml E. coli culture under standard conditions (5.0 OD600 culture in our expression conditions) in the DNA2.0 laboratory (detectable via SDS-PAGE and/or Western).
  • * There is an additional $50 USD fee per gene for Western Blot service.
  • Expression solutions are only available when using the pJExpress 400 series of T5 vectors (401, 402, 404, 406) in E. coli and certain E. coli Electra® vectors (pD441-SR and pD444-SR).
    • pJ401-T5-Kanamycin
    • pJ402-T5-Zeomycin
    • pJ404-T5-Ampicillin
    • pJ406-T5-Kan/Amp/Chloramphenicol
  • Listed prices are for genes <3kb in length. Please consult with our Expression Specialists to determine specific pricing for genes longer than 3kb.
  • DNA2.0 will provide electronic gel image and protocols for expression to the customer.
  • DNA2.0 does not guarantee protein solubility.
  • DNA2.0 does not guarantee the ability of customers, researchers or external labs to produce similar expression levels.
  • DNA2.0 reserves the right to decline Expression Test and Guaranteed Expression orders.
  • For a Guaranteed Expression gene which fails to express: DNA2.0 will credit the customer account with a $750.00 USD DNA2.0 GeneCard. The GeneCard balance can be applied to future orders with DNA2.0. There are no fees returned for proteins which do not express.

Let DNA2.0 Increase Your Expression

Consult with an Expression Specialist today at +1 877 362 8646 or info@DNA20.com to achieve your research goals quickly and affordably.