GeneGPS™ Optimizes Expression in Your System.
DNA2.0 has employed a variety of multivariate analysis strategies to identify relationships between gene design variables and protein expression. We have ongoing studies in Bacteria, Yeasts, Mammalian cells and in vivo, Plants, Fungi, Insects, and Cell-free systems. DNA2.0 is using the results of our research to create patented gene design algorithms for numerous host systems. When utilized, these new GeneGPS™ design algorithms routinely produce 10-100 times more protein than competing methods. Results from these investigations can be seen in DNA2.0 PepTalk 2014 Presentation and below.
An example, from work we have been doing in partnership with Dr. Robert Stroud at the University of California, San Francisco, shows improved expression in a yeast host system.
- Model based on total secreted enzyme activity
- Strong MeOH-induced AOX1 derived promoter
- Similar models obtained for each promoter
- No significant correlation with CAI or GC percent