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VectorsDNA-2-Go™ synthetic genes are cloned into our own series of pJ vectors. These vectors have been designed to minimize common restriction sites in the vector for ease of downstream manipulation. Inserts are flanked by transcriptional terminators to allow cloning of inserts encoding proteins that are toxic to E. coli. Vector choice is determined by several factors including the size of the insert, the construction process to be used and the antibiotic resistance required. We also have vectors with low copy origins for cloning genes that are problematic despite the transcriptional terminators. If you have specific requirements please let us know. Please note:- The pJ vectors do not contain restriction sites for excision of your gene. These must be included in your gene design if you wish to remove your gene with restriction enzymes.
- Inserts are cloned nondirectionally to minimize potential toxicity of inserts.
- Cloning into any customer-specified alternative vector or Invitrogen Gateway® Entry vector pDONR221 is also available for an additional charge. Please inquire for quote.
| Vector | Vector Size (kb) | Marker | Ori | | pJ201 | 2.7 | Kan | pUC | | pJ204 | 2.8 | Amp | pUC | | pJ206 | 5.0 | KanAmpChlorGent | pUC | | | | | | | pJ211 | 2.6 | Kan | pACYC | | pJ214 | 2.7 | Amp | pACYC | | pJ216 | 4.9 | KanAmpChlorGent | pACYC |
For PCR or sequencing primers we use the following oligonucleotides: | pTF | 5'-CTCGAAAATAATAAAGGGAAAATCAG-3' | | pTR | 5'-TGGTAGTGTGGGGACTC-3' |
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info@DNA20.com
1 877 DNA TOGO
1 650 853 8347
europe@DNA20.com |
