Expert Custom Gene DesignEveryone at DNA2.0 has considerable first-hand experience in gene synthesis and design, and will be very happy to walk you through the entire gene design process, making sure that your synthetic gene includes all of the features that you need. Enhance heterologous protein expressionBecause a synthetic gene is designed from the ground up, the codons used to encode different amino acids can be chosen to suit the expression host. Codon-optimizing your synthetic genes often results in greatly enhanced heterologous protein expression. Read more Easily modify the sequence to suit your needsCustom gene synthesis allows you to design exactly the gene you need: - Quickly add N- or C-terminal affinity tags.
- Insert linkers.
- Create fusion proteins or fusion transcripts.
- Add upstream ribosome binding sites to give optimal bacterial protein expression.
- Surround eukaryotic initiating AUG codon with optimal Kozak consensus sequence.
- Add or remove introns.
- Add or remove sites for protein modifications such as phosphorylation, glycosylation or PEGylation.
- Add or remove restriction sites to facilitate downstream gene manipulations.
Create sets of gene variantsSets of synthetic gene variants are valuable in structural biology, where proteins with slightly different starting and ending points have quite different solubility and crystallization properties. We also synthesize gene variant sets to create protein variants for our ProteinGPS™ protein engineering technology. We have developed a process for creating many specific variants of a gene at a much lower cost per gene than synthesis of a single sequence. Gene Designer softwareTo build a gene with the desired design we use the DNA2.0 Gene Designer software. It is also available for you to download, free of charge. Gene Designer is fundamentally different from other sequence manipulation programs—it is not just another sequence analysis tool. We built it to simplify the whole design process, starting with an intuitive drag-and-drop interface supported by powerful optimization algorithms. Assembling genetic building blocks using Gene Designer is easy and fun. |
